Showing posts with label Master's project. Show all posts
Showing posts with label Master's project. Show all posts
Robotic arm with a small foot-print

Master's degree/engineering course project

(The earliest starting date June 1st 2018. For more info contact Alyona)


 Fig. 1. Six days of time-lapse tracking of the root growth. Arabidopsis thaliana seedlings
 grown on a Petri dish in a plant growth chamber. 

The research in our group is focused on molecular mechanisms underpinning development of plants. It is similar to engineering when there is nobody around to explain you what all the parts are for. So, on daily basis, we break plant genes to see what they were needed for. To track plant growth properly we need a good camera system that will image plants for days or even weeks (exactly like Fig 1, but less blurry). We grow our plants in small Petri dishes within a growth cabinet and thus need the imaging platform to have small foot print.


Preliminary results:

We put together the minimal version of the imaging stage (see Fig. 2). Despite the questionable quality of the design, it was such a significant improvement of our experiments that we decided to invest into constructing a proper system. Our system is built around a Raspberry Pi computer, connected to a camera capable of producing images both under daylight conditions and at night using near-infrared illumination.
The growth cabinet
The imaging stage
Fig. 2. The current layout of the imaging stage.  The imaging takes place within the plant growth cabinet, which maintains optimal temperature and light intensity for the plants. Plants within a Petri dish are mounted on a Sugru holder and imaged by the IR camera taped to the piece of cardboard. The camera is plugged into a raspberry pi computer that saves images on a server connected via WiFi.






Our group has just installed a Prusa i3 MK3 3D printer!
In collaboration with JJ we will soon print our first prototype for time-lapse imaging  of 4 plates.

Fig. 3. Update (August 2018).  No more double-sided tape on cardboard! We are switching to 3D printing.


Project goals and requirements: 
1. Make the imaging stage less prone to falling apart. Additionally, the stage should be constructed in such a way that it provides more consistent images with regards to lighting.

2. Develop a small footprint robot that can move plates from the growth position to the imaging stage and back. This will enable parallel imaging of multiple plates, while not compromising the amount of light available to the plants.


3. The robot needs to be able to communicate with the Raspberry Pi, either via ethernet, WiFi, bluetooth or serial/GPIO (or you may have other suggestions). 



Why should you join us:

Fig 3. The team.
1. You will work in a fantastic team (see Fig. 3). We love what we do, and call it "work" just because we get paid for it.

2.  You will co-author an open access publication describing the hardware and software of the system, helping other scientists to build on what we develop. All blueprints and software packages will be made available on-line under a permissible open source license.

3. We will introduce you to engineering on the DNA level.


Master's degree project in plant genetic engineering. 

Available earliest from the September 20th 2018.


Contact:
 Alyona Minina, PhD: alena.minina (@) slu.se
 Anna Åsman, PhD: anna.asman (@) slu.se


In our group we are currently focusing on investigating the molecular machinery of plant autophagy. We are looking for a highly motivated student who is interested in  joining our group to optimize CRISPR-Cas9 system for knock-in modification of plant genes. 

An example of CRISPR-Cas9 driven knock-in.
A stop codon of a gene in Arabidopsis genome
is replaced with a DNA sequence encoding the
Green Fluorescent Protein (GFP). The resulting
plant expresses a GFP fusion of the endogenous
protein.

Most of the current plant molecular biology studies still rely on the use of crude genetic engineering tools that dramatically limit the capacity of our research. The recent advances in the use of CRISPR-Cas9 system for plants give very promising results that still require some significant modifications. 


In this project we aim to optimize the CRISPR-Cas9 for precise knock-in modification of Arabidopsis genes and use the new tool to make reporter lines for detection of plant autophagy-related (ATG) genes activity. 


This project, in general, will open up a broad range of new possibilities for investigating plant gene function and in particular, will make a significant contribution to our understanding of ATG-genes regulation.  



Project goals
  1. Establish proof of concept constructs for knock-in modification of Arabidopsis thaliana genes in protoplasts
  2. Create a set of constructs for knock-in modification of genes important for regulation of autophagy in Arabidopsis thaliana
  3.  Participate in establishing transgenic lines by knocking in green fluorescent protein and luciferases into Arabidopsis genome


You will acquire skills in
  1. Genetic engineering
  2. Use of CRISPR-Cas9 in plants
  3. Advanced DNA and protein molecular biology methods
  4. Advanced confocal microscopy
  5. Plant transformation
  6. Handling typical plant model organisms: Arabidopsis thaliana plants and tobacco cell cultures


Master's degree project in plant cell molecular biology

Available earliest  March 1st 2019. 

Contact:
Alyona Minina, PhD: alyona.minina(at)slu.se
Adrian Dauphinee, PhD: adrian.dauphinee(at)slu.se



Flyer

Detection of autophagy activity changes
 in epidermal root cells of Arabidopsis thaliana


Autophagy is the major catabolic process of eukaryotes allowing cells to recycle their own contents. It is intensively investigated by plant biologists to elucidate mechanisms regulating plant fitness and stress tolerance. Development of precise molecular tools to study plant autophagy is a difficult but an extremely important task.


In this project we aim to study in details the effects of a drug typically used to modulate autophagy in plant cells. The project is based on strong preliminary data indicating that the effect of the drug might be much more complex than usually assumed.



Project goals:
  1. Quantification of successive changes in autophagy activity during the drug treatment using advanced fluorescent microscopy and biochemistry methods
  2. Investigating activity of potential off-targets of the drug
  3. Optimization of the drug concentration and treatment duration for minimizing possible side effects



 You will acquire skills in:
  1. Advanced fluorescent microscopy 
  2. Handling typical plant model organism Arabidopsis thaliana 
  3. Advanced DNA and protein molecular biology methods 
  4. Genetic engineering

Posted by Alyona Minina on the 2018.11.06.

Master's degree project in plant cell molecular biology

COS, Heidelberg University, Germany
Available earliest from the February 1st 2019. 

Contact:
Alyona Minina, PhD: alyona.minina (@) cos.uni-heidelberg.de
Jana Askani, MSc: jana.askani (@) cos.uni-heidelberg.de
Karin Schumacher, Prof:  karin.schumacher (@) cos.uni-heidelberg.de

Flyer

Autophagy is the major catabolic process underpinning sustainability of eukaryotic cells. It is the process by which cells engulf cargo destined for degradation into double-membrane vesicles, autophagosomes, deliver them to a lytic compartment (vacuole) and upcycle the products of degradation.



Tracking autophagosomes in epidermal root cells of Arabidopsis thaliana.




Elucidating the molecular machinery of plant autophagy enables understanding of how plants cope with biotic and abiotic stresses and contributes to our knowledge of plant developmental programs.
In our group we combine a unique expertise in plant vacuole biogenesis, endomembrane trafficking and autophagy. In this project we are going to investigate key steps of plant autophagosomes maturation prior to their fusion with the vacuole.



 Project goals:

  1. Establishing a set of transgenic lines expressing mutant forms of proteins required for plant autophagosomes maturation
  2. Detecting dynamics of autophagy efficacy in the established lines
  3. Planning and making genetic constructs for further mutations to elucidate the maturation mechanism in more details



 You will acquire skills in:
  1. Advanced fluorescent microscopy 
  2. Handling typical plant model organism Arabidopsis thaliana 
  3. Genetic engineering 
  4. Advanced DNA and protein molecular biology methods 
  5. Plant transformation 


Posted by Alyona Minina on the 2018.11.06.